Stability and activity of lactate dehydrogenase on biofunctional layers deposited by activated vapor silanization (AVS) and immersion silanization (IS)
نویسندگان
چکیده
منابع مشابه
Covalent attachment of cholesterol oxidase and horseradish peroxidase on perlite through silanization: activity, stability and co-immobilization.
In the present work, co-immobilization of cholesterol oxidase (COD) and horseradish peroxidase (POD) on perlite surface was attempted. The surface of perlite were activated by 3-aminopropyltriethoxysilane and covalently bonded with COD and POD via glutaraldehyde. Enzymes activities have been assayed by spectrophotometric technique. The stabilities of immobilized COD and POD to pH were higher th...
متن کاملOmniphobic RF Paper Produced by Silanization of Paper with Fluoroalkyltrichlorosilanes
The fabrication and properties of “fl uoroalkylated paper” (“R F paper”) by vapor-phase silanization of paper with fl uoroalkyl trichlorosilanes is reported. R F paper is both hydrophobic and oleophobic: it repels water ( θ app H 2 O >140°), organic liquids with surface tensions as low as 28 mN m −1 , aqueous solutions containing ionic and non-ionic surfactants, and complex liquids such as bloo...
متن کامل“ Omniphobic “ RF Paper ” Produced by Silanization with Fluoroalkyltrichlorosilanes ”
F) were purchased from Gelest Inc (Morrisville, PA). All chemicals were used as received without further purification. Paper (Gel Blot paper, Whatman#1, and Whatman#50) was purchased from GE Healthcare (NJ, USA) and used as received, without purification. The silanization reaction was conducted in a chamber with a volume of 0.01 m 3 at a temperature set at 95 °C. The silanizing reagent is trans...
متن کاملantioxidant and antimicrobial activity of allium jesdianum extract
چکیده بررسی فعالیت ضد اکسیدانی و ضدمیکروبی عصاره گیاه بن سرخ (allium jesdianum) به کوشش: زهرا مقیمی هدف از این تحقیق بررسی میزان ترکیبات فنولی کل، ترکیبات فلاوونوئیدی کل، فعالیت ضداکسیدانی و ضدمیکروبی عصاره ی گیاه تازه و خشک بن سرخ بود. استخراج عصاره به روش های غوطه وری، استفاده از همزن مغناطیسی، دستگاه مایکروویو، امواج فراصوت و گرمادهی مقاومتی و با استفاده از متانول، اتانول و آب به عنوا...
VARIATIONS BY EPINEPHRINE OF HEPATIC AND SERUM AMINOTRANSFERASES AND LACTATE DEHYDROGENASE IN THE RAT
Incubation of rat hepatocytes with epinephrine inhibited alanine aminotransferase (ALT) (80%) and aspartate aminotransferase (AST) (53%) activities with no effect on lactate dehydrogenase (LDH) activity. Injection of epinephrine caused a progressive increase with time in hepatic LDH activity, being 52% at 24 h. Preinjection with propranolol eliminated the hormone effect and caused further ...
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ژورنال
عنوان ژورنال: Applied Surface Science
سال: 2017
ISSN: 0169-4332
DOI: 10.1016/j.apsusc.2017.04.123